MSX2 Induces Trophoblast Invasion in Human Placenta
Fig 7
MSX2 is significantly downregulated in the placental villi from PE patients.
(A) Immunostaining of MSX2 in the EVTs from normal and PE placentas at similar gestational age (a and b). CK7 was used as a marker for EVTs (c and d). Negative control images (NEG) in which normal IgG was used to replace the primary antibody showed no specific positive staining (e and f). Boxed region is enlarged on the under panel. Scale bar = 200μm. NP: nomal term placenta PE: pre-eclampsia (B) Western blot analysis of MSX2 expression in the human placental villi from term placentas (38 weeks; n = 3) and PE placentas (38 weeks; n = 3). (C) Results obtained in B were quantified by measuring the protein band intensity of MSX2 relative to that of GAPDH (n = 8; t-test; **P < 0.01).