MSX2 Induces Trophoblast Invasion in Human Placenta
Fig 2
Overexpression and siRNA-mediated knockdown of MSX2 results in enhanced and reduced HTR8/SVneo cell invasion, respectively.
(A) Overexpression of MSX2 induces invasion in HTR8/SVneo cells. (a) Representative images of filters containing invaded cells from Matrigel invasion assays. (b) Statistical bar graphs showing the averaged results from three independent experiments (t-test). (c) Examination of MSX2 overexpression efficiency after transfection. And statistical assay of the results in (d) (t-test; *P < 0.05). (B) siRNA-mediated knockdown of MSX2 expression inhibits HTR8/SVneo cell invasion. (a) Representative images of filters containing invaded cells from Matrigel invasion assays. (b) Statistical bar graphs showing the averaged results from three independent experiments. (t-test) (c) Examination of MSX2 knock down efficiency after siRNA transfection. And statistical assay of the results in (d) (t-test; **P < 0.01; n = 3). GAPDH was used as a loading control.