The Translocation Domain of Botulinum Neurotoxin A Moderates the Propensity of the Catalytic Domain to Interact with Membranes at Acidic pH
Fig 6
Comparison of permeabilization of, and membrane binding to LUV by LC, HN and LC-HN proteins of BoNT/A.
A, B, C, Kinetics of SRB leakage from LUV (100 μM) after addition of LC (A), HN (B) and LC-HN (C) (0.1 μM) at pH 7 (black), pH 6 (pink), pH 5 (green), pH 4.5 (orange), pH 4 (blue) and pH 3.5 (red). S3 Fig shows absence of SRB leakage in the absence of proteins and 100% SRB release after Triton X-100 addition. D, FRET from LC (red), HN (green) and LC-HN (blue) at 0.5 μM to dansyl-DHPE-containing LUV at 50 μM.