The Translocation Domain of Botulinum Neurotoxin A Moderates the Propensity of the Catalytic Domain to Interact with Membranes at Acidic pH
Fig 4
Effect of pH on the stability of LC, HN and LC-HN proteins. GdnHCl-induced denaturation of LC, HN and LC-HN followed by Trp fluorescence at pH 7 and pH 4.
A, B, C, denaturation curves of LC, HN and LC-HN respectively, at pH 7 (open symbols) and pH 4 (closed symbols). D, superposition of the denaturation curves of LC (red squares), HN (green circles) and LC-HN (blue triangles) at pH 4. Inset, table of thermodynamic parameters ΔG0 and D1/2, for each protein at pH 7 and pH 4.