Macrophage-Colony Stimulating Factor Derived from Injured Primary Afferent Induces Proliferation of Spinal Microglia and Neuropathic Pain in Rats
Fig 3
M-CSF receptor mRNA robustly induced in spinal microglia after peripheral nerve injury.
PCR products from the spinal cord (L4-5) taken from 0 (naive), 0.5, 1, 2, 3, 7, and 14 days after SNI. Representative gel panels are shown in A. M-CSFr mRNA levels was normalized against GAPDH (n = 4, mean ± SEM; #, p < 0.05 compared with naive). Darkfield ISHH-images show the expression of M-CSFr mRNA (B, D) in spinal cord of naive (B) and 3 days after nerve injury (D). (C, E) Higher magnification brightfield images of dorsal horn of the left-hand photographs. Sections were counterstained by H-E. (F-H) Double labeling analysis of M-CSFr mRNA at 3 days after SNI. Photographs show combined ISHH for M-CSFr with NeuN (F), GFAP (G) and Iba1 (H) of the spinal dorsal horn at 3 days after SNI. Arrowheads indicate single-labeled cells by ISHH (aggregation of grains), and open arrowheads indicate single immunostained cells (brown staining). Sections were counterstained by hematoxylin. Arrows indicate double-labeled cells. Scale bar = 500 μm in B, D; 25 μm in C, E-H. contra, contralateral; ipsi, ipsilateral.