GSK-3α Is a Novel Target of CREB and CREB-GSK-3α Signaling Participates in Cell Viability in Lung Cancer
Fig 1
CREB inhibition suppresses the expression of GSK-3α.
(A) Effect of CREB knockdown on the mRNA level of GSK3A, GSK3B, and CREB. Each of the indicated cells were transfected with control siRNA or CREB siRNA (40 nM, each) for 48 h, followed by qPCR analysis. All values in the graphs represent mean ± SD of three independent experiments. Two-sided t-test. *, P < 0.01. (B-C) Effect of CREB knockdown on the protein levels of GSK-3α (B) and GSK-3β (C). Each of the indicated cells were transfected with control siRNA or CREB siRNA (40 nM, each) for 72 h, followed by western blot analysis.