Interleukin 6 Accelerates Mortality by Promoting the Progression of the Systemic Lupus Erythematosus-Like Disease of BXSB.Yaa Mice
Fig 2
B cells from BXSB.Yaa mice are potent producers of IL6.
(A) CD43- B cells were purified from spleen, BM and peritoneal lavage of 4–5 month old BXSB.Yaa and BXSB mice and stimulated with graded concentrations of Imiquimod (R837). (B) Dendritic cells enriched from spleen (splenic DCs) or differentiated from BM precursors (GMCSF (40ng/ml) and IL4 (20ng/ml) for 9 d with media changed every third day), peritoneal macrophages and CD115+ monocytes from spleens of BXSB.Yaa and BXSB mice were stimulated with R837 (50ng/ml) in vitro. Supernatants were collected after 24 hours and IL6 levels were quantified using a standard sandwich ELISA. Data expressed as mean ± SEM of triplicate wells are representative of 4–6 independent experiments. p values are determined by two-way ANOVA. * p<0.05, ** p<0.01, ***p<0.0001.