Identification of a Tumor Specific, Active-Site Mutation in Casein Kinase 1α by Chemical Proteomics
Fig 2
Analysis of a CSNK1A1 active-site peptide in both colon tumor and matched control samples.
A) Amino acid sequence of the wild type peptide. B) LC-MS/MS spectra for the CSNK1A1 peptide. The colon tumor sample is off-set in the Y-axis by 50 units for clarity and the region of interest (ROI) is highlighted. C) Region of interest in the LC-MS/MS spectra for colon tumor and matched control samples. Each spectrum is normalized to the highest intensity ion. D) Extracted ion chromatograms for CSNK1A1 from both colon tumor and matched control samples. Signals are normalized to the peak eluting at 59.8 minutes.