Diverse Basis of β-Catenin Activation in Human Hepatocellular Carcinoma: Implications in Biology and Prognosis
Fig 1
Regulation of β-catenin target genes by CTNNB1 exon3 mutation in human HCC cell line.
A. Stable cell line expressing CTNNB1 exon3 mutation (Hep3B-S33Y) is established. Control cell line is shown as Hep3B-pCI. Immunofluorescent staining is performed with β-catenin antibody. Nuclear staining is performed with Hoechst33342. Arrow heads show nuclear translocation of β-Catenin. B. TOP-Flash activity is compared between Hep3B-S33Y and Hep3B-pCI. C. β-catenin target protein expressions (GS, Regucalcin and Lect2) of Hep3B-S33Y and Hep3B-pCI are shown by Western blot.