Intercellular Protein Transfer from Thymocytes to Thymic Epithelial Cells
Fig 3
Contribution of TCR-MHC engagement dependent and independent mechanisms to protein transfer from thymocytes to TECs.
A, B. Frequencies (A) and absolute numbers (B) of TECs in TCRα-/-ZsGreeen-CD4Cre and TCRα+/-ZsGreeen-CD4Cre mice. C. Representative dot plots of showing mTEC and cTEC subsets. D, E. Percentages (D) and absolute numbers (E) of mTECs and cTEC. F. Overlaid histograms showing ZsGreen level in mTECs and cTECs. G. Relative gMFI of ZsGreen in m/cTECs. gMFI in TCRα+/-ZsGreeenf/+CD4Cre TECs was arbitrarily set as 1. H. ZsGreen intensity of CD45+EpCAM-CD4+CD8+ DP thymocytes. Data shown are representative or calculated from three experiments. *, p<0.05 determined by Student t-test. Raw data for Fig 3A, 3B, 3D, 3E, and 3G are shown in S1 Data.