Selenoprotein Transcript Level and Enzyme Activity as Biomarkers for Selenium Status and Selenium Requirements of Chickens (Gallus gallus)
Fig 5
Effect of dietary Se on significantly-regulated selenoprotein transcript level in chick liver.
Relative transcript levels are plotted for GPX1 (A), GPX3 (B), GPX4 (C), DIO1 (D), SELH (E), SELM (F), SELU (G) and SEPP1 (H) in chicks supplemented with the indicated levels of dietary Se for 29 d. Values were determined in triplicate for each sample, normalized to the mean of GAPDH and ACTB levels in each sample, expressed as a percentage of Se-adequate plateau levels, and plotted as mean±SEM (n = 4/treatment). Means without a common letter are significantly different (P<0.05). Overall level of significance, as determined by ANOVA, is given in Table 3. Se response curve breakpoints (BP) are indicated in each panel, calculated as described in the text.