A Rapid and Improved Method to Generate Recombinant Dengue Virus Vaccine Candidates
Fig 2
RNA transcripts from LONG-PCR template.
In vitro transcription reactions were performed using DNA templates from single-plasmid or two-plasmid (ligation/non-PCR and ligation/LONG-PCR) constructs. Equal volume aliquots of RNA transcripts produced were denatured at 500 C for 30 minutes and then were electrophoresed on a 1% denaturing glyoxal gel. Control lane indicates RNA transcript produced from the control DNA template included in the in vitro transcription kit.