TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5)
Fig 4
IL-7-induced STAT5 phosphorylation is down regulated in Gimap5-deficient T cells.
(A) CD4+ T cells were purified from freshly isolated lymphocytes from wild type and Gimap5sph/sph mice. Equal amounts of protein lysates were subjected to Western blot analysis for the expression of FOXO1 and pFOXO1. Data shown are representative of 3 independent experiments. (B) Thymocytes and lymph node cells were stained with antibodies to CD4 and CD8. CD127 expression was assessed in gated CD4+ SP or CD8+ SP thymocytes and CD4+ or CD8+ T cell subsets by flow cytometry. Data shown are representative of 3 independent experiments. (C) Purified CD4+ T from Gimap5sph/sph mice or Gimap5lyp/lyp rats were stimulated with IL-7 or IL-15 at 37°C. At the indicated time points the cells were lysed and subjected to Western blot analysis for the detection of phosphorylated STAT5, total STAT5 or Bcl-2. Lower panel represents the densitometric data pooled from 3 independent experiments. * p value < 0.05.