Holothurian Nervous System Diversity Revealed by Neuroanatomical Analysis
Fig 6
Labeling by neuronal markers of transverse sections of the radial nerve cord identify the peripheral nerves.
In a transverse section, the ectoneural peripheral nerve extends from the ectoneural component of the RNC into the dermis (DE) to form the podial nerve (arrow). Immunoreactivity within the ectoneural peripheral nerves was observed with the RN1 antibody (A), anti-calbindin (B), and anti-pax6 (C). In a transverse section, the hyponeural peripheral nerve extends from the hyponeural component of the RNC into the longitudinal muscle (CM) (arrowhead) and longitudinal muscle (LM) (double arrowhead). Immunoreactivity within the hyponeural peripheral nerves was observed with the RN1 antibody (D), anti-calbindin (E), and anti-pax6 (F). Scale bar = 40 μm in C, and E, 150 μm in A, B, D, and F.