Oral Administration of p-Hydroxycinnamic Acid Attenuates Atopic Dermatitis by Downregulating Th1 and Th2 Cytokine Production and Keratinocyte Activation
Fig 4
Oral delivery of p-hydroxycinammic acid changes the size and weight of immunological organs and downregulates the production of immunoregulatory cytokines by the draining lymph nodes of mice with atopic dermatitis.
The four mouse groups are described in the legend to Fig 1. (A) Representative photographs showing the size and weight of the draining lymph nodes (dLN) and spleens of the four mouse groups 28 days after atopic dermatitis (AD) started. The smallest gradation on the ruler is millimeter. The data of the groups (n = 3–6/group) are shown as mean ± SD. *P<0.05 versus the AD control group. (B, C). Gene expression of cytokines in CD4+ T cells from dLNs (B) and non-dLNs (C) that were harvested on day 28. The CD4+ T cells in the lymph nodes were purified by magnetic activated cell sorting according to the manufacturer’s instructions, total RNA was isolated, and the expression of the indicated cytokine-encoding genes was measured by real-time RT-PCR. The data are presented as the mean ± SD (n = 3–6/group). *P<0.05, versus the AD control group.