Effect of Aminated Mesoporous Bioactive Glass Nanoparticles on the Differentiation of Dental Pulp Stem Cells
Fig 2
Cell viability after 24 h incubation with various concentrations of MBNs–NH2 in (a) undifferentiated or (b) differentiated rDPSCs. After cells were plated at a volume of 100 μL of 105 cells per well on a 96-well plate for 24 h, 5, 7.5, 10, 12.5, 15, 20, 25, 50, 75, 100, 200, or 400 μg MBNs–NH2 per mL of initial 105 rDPSCs (0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 5, 7.5, 10, 20, or 40 μg MBNs–NH2 to 100 μL of 104 rDPSCs) was incubated in each well of a 96-well plate for an additional 24 hours with conventional supplemented media or odontogenic supplemental media. After 1 day, cell viability was determined using water-soluble tetrazolium based on the percentage of the optical density (450 nm) value in the experimental group versus the non-treated control. Assays were independently performed in triplicate. Representative averages (n = 5) and SD were recorded. * Significant difference vs. non-treated control (p<0.05).