An Oxalyl-CoA Dependent Pathway of Oxalate Catabolism Plays a Role in Regulating Calcium Oxalate Crystal Accumulation and Defending against Oxalate-Secreting Phytopathogens in Medicago truncatula
Fig 2
Measurement of oxalate degradation to CO2.
(A) Schematic of the proposed pathway of oxalate catabolism in M. truncatula. Asterisks denote enzymatic steps remaining to be validated. (B) Radiolabeled CO2 measurements. WT and Mtaae3 knock-down leaf pieces were fed with 2.5 μCi of [14C]-oxalate along with 300 μM non-labeled oxalate. The 14CO2 evolved was captured using 1M KOH and the relative radioactivity was measured. (C) Relative MtAAE3 transcript levels in leaves of Mtaae3 knock-down lines compared to WT as measured by qRT-PCR. Ubiquitin was used as the reference gene.