Hexarelin Protects Rodent Pancreatic Β-Cells Function from Cytotoxic Effects of Streptozotocin Involving Mitochondrial Signalling Pathways In Vivo and In Vitro
Fig 2
Hex reduced STZ-induced mitochondrial damage and in pancreatic cells.
The MIN6 and α-TC6 cells were treated with 1.0 mM STZ and 1.0 μM Hex alone or in combination. Cell viability of (A) the MIN6 and (B) α-TC6 cells was measured by MTS assay (n = 6). The MIN6 cells were then treated with 1.0 mM STZ and 1.0 μM Hex alone or in combination. (C) The changes in the mitochondrial potential of the MIN6 were determined by Rhodamine 123 assay. (D) Cell superoxide activity of the MIN6 was detected by superoxide DHE production. The data are presented as the mean ± SEM (n = 8). *p < 0.05, compared with the control group; #p < 0.05, ##p < 0.01, compared with the STZ group.