Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4
Fig 6
Pretreatment with Pam3CSK4 increases antimicrobial activity of neutrophils.
Neutrophils were purified according to the instructions (Miltenyi,Gemany). Neutrophils were purified and measured by flow cytometry (A), and PE/CY5.5-Rat IgG2b(κ), PE-anti Rat IgG 2b(κ) as isotype controls(left panel in Fig 6A). The bacterial cfu of neutrophils was calculated (B), the left panel (the medium contol) indicated the well including MRSA and medium (RPMI1640) but Pam3CSK4 and neutrophils. FITC-positive neutrophils were quantified by flow cytometry(C). Survival of mice injected with neutrophils co-cultured with Pam3CSK4, and previously inoculated with MRSA 1 hour earlier (D). Generation of ROS was measured by flow cytometry (E) and lactoferrin release was tested using mouse Lactoferrin ELISA Kit (F). Note: *P<0.05; **P<0.01 versus normal saline-treated mice.