Vitamin D Supplementation Reduces Induction of Epithelial-Mesenchymal Transition in Allergen Sensitized and Challenged Mice
Fig 2
Effect of vitamin D on total and differential leukocytes in BALF of PBS and HRA-sensitized mice.
Total cells in the BALF were counted using Countess Automated Cell Counter and differential analysis was performed using standard morphological criteria. A minimum of 300 cells were examined per cytospin slide and absolute cell numbers for each cell type were calculated according to the total leukocyte counts in BALF and the percentage of each individual cell type on the slide. Data are shown as mean ± SEM for seven animals in each group (*p < 0.05; **p < 0.01; ***p < 0.001).