Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies
Fig 5
TaEDC4 accumulates in P-bodies.
(A) Schematic representation of the protein primary structure of AtEDC4, NbEDC4, and TaEDC4. Numbers indicate amino acid positions. The percentage of pairwise amino acid sequence identity is indicated to the right of the diagram. (B) Live-cell imaging of TaEDC4-mCherry and YFP-VCSc in N. benthamiana leaf cells. Images show a single optical section of 0.8 μm. Proteins were transiently expressed in N. benthamiana leaf cells by agroinfiltration. Live-cell imaging was performed with a laser-scanning confocal microscope with a sequential scanning mode two days after infiltration. The YFP was excited at 514 nm; mCherry and chlorophyll were excited at 561 nm. YFP (yellow), mCherry (red), and chlorophyll (blue) fluorescence were collected at 525–550 nm, 580–620 nm, and 680–700 nm, respectively. White arrowhead: nuclei; black arrowhead: P-bodies. The intensity plot in the top right corner shows YFP and mCherry (RFP) relative fluorescence signal intensity along the white line connecting points a and b in the overlay image.