Genome-Wide Chromatin Landscape Transitions Identify Novel Pathways in Early Commitment to Osteoblast Differentiation
Fig 4
Heatmap of microarray analysis.
(A) Heatmap of expression for top 135 genes with at least 5-fold change in expression was created using heatmap.2 in R program. These genes were separated into 6 major groups. Complete list of these genes with fold change can be found in S5 Table. Expression for each gene is calculated as difference from control values. All data represents means of at least three independent biological replicas. (B) Top networks identified by IPA represented by the 6 groups as shown in the heatmap. Dexamethasone was selected among several top upstream regulators indicating additional value of using this type of analysis. The scores for Dexamethasone were assigned by IPA program and is shown on the last column.