A Comparative Study of Variables Influencing Ischemic Injury in the Longa and Koizumi Methods of Intraluminal Filament Middle Cerebral Artery Occlusion in Mice
Fig 3
Post-reperfusion time-course of ischemic lesion volume detected by TTC following 60 min intraluminal filament MCAO using the Koizumi method
(A) Representative TTC stained brain sections indicating areas of healthy tissue (red) and ischemic injury (white) for each group. (B) Total volume of ischemic lesion in the ipsilateral hemisphere, expressed as a percentage of the total contralateral hemisphere volume, 30 min, 4 h, 12 h and 24 h after reperfusion following sham surgeries or 60 min MCAOs, with thin or thick silicone coated filaments, via the Koizumi method. (C) No alteration in neurological severity scores between animals assessed at 4 h, 12 h and 24 h after reperfusion, following a 60 min occlusion. (D) Body weight loss post-MCAO significantly increased from 24 h as compared to 4 h and 12 h after reperfusion. Each value represents the mean ± the standard error of the mean (SEM). *p < 0.05. N = 3–4 for sham surgeries and n = 5–8 for animals undergoing occlusion.