Structural Analysis and Deletion Mutagenesis Define Regions of QUIVER/SLEEPLESS that Are Responsible for Interactions with Shaker-Type Potassium Channels and Nicotinic Acetylcholine Receptors
Fig 1
Structural modeling of SSS loop deletion mutants.
(A) Amino acid sequence of SSS minus the predicted cleaved N- and C-termini. Loop deletions are designated by green boxes. Predicted N-linked glycosylation site (N57) is highlighted in purple and the predicted GPI-anchor attachment site (N127) is highlighted in light blue. (B) De novo Robetta model of SSS-WT. The N-terminus (after cleavage of the signal peptide) is marked accordingly. Red sticks indicate predicted disulfide bonds. Loop regions are denoted in navy (loop 1), orange (loop 2) and green (loop 3). For all panels, deleted protein segments are indicated with a dashed line.