Competitive Mirror Image Phage Display Derived Peptide Modulates Amyloid Beta Aggregation and Toxicity
Fig 11
Analysis of potential γ-secretase activity alterations by Mosd1.
Detection of CTFβ and β-actin by enhanced chemoluminescence on a Western blot of lysed human APP695-transfected Neuro-2a cells. Human APP695-transfected Neuro-2a cells were grown in 24-well plates for 24 hours. Cells were incubated with DMSO (lane 1), DAPT (lane 2), 10 μM Mosd1 (lane 3) or 100 μM Mosd1 (lane 4) for additional 24 hours. The cells were harvested, lysed and proteins were separated by Tris-tricine SDS-PAGE. Proteins were blotted on a PVDF membrane and detected with an anti-APP-CTF antibody as well as an anti-β-actin antibody. Binding of HRP conjugated secondary antibodies was detected by transformation of enhanced chemoluminescence (ECL) substrate by HRP.