Serum Amyloid A Induces Toll-Like Receptor 2-Dependent Inflammatory Cytokine Expression and Atrophy in C2C12 Skeletal Muscle Myotubes
Fig 5
SAA causes reduction in myotube widths that can be reversed by blocking TLR2.
(A) Control (left panel) and SAA 1μg/ml (right panel) C2C12 myotubes treated with SAA for 48h, immunolabelled with anti-desmin antibody (green). Scale bar = 100μm. (B) Quantification of myotube widths from immunofluorescence images. (C) Myotube widths in C2C12 myotubes treated with TLR2 neutralising antibody prior to SAA stimulation for 48h. Data shown are mean ± SEM for n = 3 independent experiments, statistical significance shown relative to control myotubes (0μg/ml SAA), * p<0.05.