Monoclonal Antibody RYSK173 Recognizes the Dinuclear Zn Center of Serum Carnosinase 1 (CN-1): Possible Consequences of Zn Binding for CN-1 Recognition by RYSK173
Fig 2
Recognition of recombinant and serum CN-1 by RYSK173.
A: recombinant CN-1 was expressed in HUVECs by lentiviral transduction. The recombinant proteins were detected in cell lysates and supernatants using the ATLAS (filled bars) and RYSK173 (open bars) based ELISA. The results of 6 transduction experiments are depicted and expressed as mean CN-1 concentration (ng/ml) ± SD. B: Serum samples of 111 healthy individuals were tested in the ATLAS and RYSK173 based ELISA. C: Cell disruption was performed by repeated freeze thawing. Similar as in serum RYSK173 detected significantly lower amounts of CN-1.