Remote Ischemic Postconditioning Ameliorates the Mesenchymal Stem Cells Engraftment in Reperfused Myocardium
Fig 5
MSCs retention in myocardium and cardiac function detection.
Cell retention by anti-brdu antibody immnohistochemical examination(A-F) and PCR quantification for SRY gene(G), and cardiac function detection(H) at 3 weeks after mesenchymal stem cells(MSCs) intramyocardial delivery (n = 8). The MSCs retention was least in sham group (p<0.05). More MSCs was calculated in RIPoC+IPoC, RIPoC and IPoC group than that in IR group (p<0.05). More retention after NAC administration compared with that in IR group, but lower retention than that in RIPoC+IPoC, RIPoC and IPoC group. LVESD was smaller in RIPoC+IPoC, RIPoC and IPoC group than that in IR group (p<0.01). LVFS was higher in RIPoC+IPoC, RIPoC and IPoC group than that in IR group (p<0.01). However, NAC administration didn’t significantly enhance cardiac function. The cells with brown-stained nuclei indicated the Brdu-labeled positive MSCs (Fig 5A). Bar indicated 100 um. The magnification was ×400 (n = 4). # indicated p>0.05, * indicated p<0.05. Group A: RIPoC+IPoC group, received the combined algorithms of RIPoC and IPoC; Group B: RIPoC group, which received 3 cycles of 30 seconds reperfusion and re-occlusion on the limb at the onset of reperfusion; Group C: IPoC group, received 3 cycles of 30 seconds reperfusion and re-occlusion on the LAD; Group D: IR group, received reperfusion without interruption after ischemia; Group E: sham group, received only open chest; Group F: NAC+IR group, ROS scavenger N-acetylcyseine was administered in IR group.