Expression of Nitric Oxide-Transporting Aquaporin-1 Is Controlled by KLF2 and Marks Non-Activated Endothelium In Vivo
Fig 1
AQP1 is preferentially expressed in endothelium overlying plaque-free intimae and is induced by KLF2.
(A, B) Heatmaps of the top 25 ranking genes as determined by GSEA. (A) Gene expression in endothelium overlying plaque-free (PF) intimae from human large arteries is compared to gene expression in endothelium from early- and advanced (ADV) lesions. Data set from Volger et al. [2]. Aquaporin 1 (AQP1) is identified as the highest ranking membrane-expressed gene in plaque-free lesions (green rectangle). (B) Time courses (24, 48 and 72 hours) of gene expression in mock-transduced HUVEC (control, c) and KLF2-transduced HUVEC are compared. Data set from Boon et al. [7]. The position of AQP1 is indicated by a green rectangle. (C) Correlation of KLF2—and AQP1 transcript levels during time courses (24, 48 and 72 hours) of gene expression in mock- and KLF2- transduced HUVECs (same data set as used for Fig 1B).