Infiltration Pattern of Blood Monocytes into the Central Nervous System during Experimental Herpes Simplex Virus Encephalitis
Fig 4
Evaluation of blood-brain barrier integrity in chimeric C57BL/6 mice following HSV-1 infection.
Micrographs illustrating the detection of albumin in the brain parenchyma of non-infected (NI) animals and mice sacrificed on days 6 and 8 following HSV-1 infection. Brain slices were processed for immunohistochemistry with primary goat anti-albumin and secondary Alexa 594-conjugated chicken anti-goat (red) antibodies. Pictures selected showed no immuno-reactivity for albumin in non-infected mice in the olfactory bulb and hindbrain. Positive control corresponds to a slice of an ischemic brain tissue obtained in a mouse model of stroke. Scale bar 400 μm.