Interference of the Histone Deacetylase Inhibits Pollen Germination and Pollen Tube Growth in Picea wilsonii Mast
Fig 4
Treatment of Picea wilsonii pollen tubes with inhibitors of HDAC induces the redistribution of actin filaments.
(A) Pollen tubes cultured in standard medium for 24 h showing actin filaments parallel to the direction of pollen tube elongation. (B) The addition of 0.2% DMSO to the standard medium followed by incubation for 24 h had no obvious effect on the distribution of the actin filaments compared with the controls. (C) In pollen tubes treated with 0.5 μM TSA-containing medium for 24 h, the actin filaments became obviously twisted and irregularly arranged. (D) In pollen tubes cultured in 0.5 mM NaB-containing medium for 24 h, the actin filaments were broken and disorganized. Bar = 20 μm.