Exposure to 17β-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida
Fig 7
Examination of E. fetida cross sections after E2 exposure.
H&E-stained cross sections of E. fetida exposed to E2 (100 μg/L), collected in the (A) 0th (control), (B) 1st, (C) 3rd, (D) 5th and (E) 8th weeks of the experiment. The collected individuals were scanned (a) and employed for the MALDI-IMS analysis of (b) the final metabolites of E2—[(E2)-3,4-Q + H]+ at m/z 287.36 Da ± 0.05%, (c) [PC2 and PC3+ H]+ merge at m/z 541.61 Da ± 0.05% (red) and 773.91 Da ± 0.05% (green), respectively, (d) [GSSG + H]+ at m/z 613.64 Da ± 0.05% and (e) [MT1 + H]+ at m/z 4798.00 Da ± 0.05% (red) and [MT2 + H]+ at m/z 7412.01 Da (green). Matrix HCCA was used. The following conditions were used to acquire the MALDI spectra: 500 shots per raster spot, 45% laser energy and 50-μm spatial resolution. The length of scale bar is 500 μm.