Reduced Levels of Proteasome Products in a Mouse Striatal Cell Model of Huntington’s Disease
Fig 1
Measurement of proteasome activity in Q7Q7, Q7Q111 and Q111Q111 cells.
Cell extracts were incubated with fluorogenic peptide substrates for 1 hour at 37°C, following which enzyme activity was determined by fluorescence measurement of AMC. The activity in Q7Q111 and Q111Q111 cells is expressed as percent enzyme activity relative to the Q7Q7 cells. Activities of the chymotrypsin-like (β5) and trypsin-like (β1) proteolytic subunits were measured by cleavage of Suc-Leu-Leu-Val-Tyr-AMC (A) and Ac-Arg-Leu-Arg-AMC (B) respectively. The error bars show standard error of mean (n = 10 for Suc-Leu-Leu-Val-Tyr-AMC and n = 5 for Ac-Arg-Leu-Arg-AMC). Statistical analysis was performed using Student’s t-test: *, p ≤ 0.05; ns, no significant difference (p > 0.05) versus Q7Q7 cells.