Differentially Expressed in Chondrocytes 2 (DEC2) Increases the Expression of IL-1β and Is Abundantly Present in Synovial Membrane in Rheumatoid Arthritis
Fig 5
Silencing of DEC2 in human fibroblasts reduces IL-1β expression after TNFα stimulation.
After 12h incubation in medium containing 1% FBS, DEC2 was silenced using Thermo Scientific On-Targetplus SMART pool DEC2 siRNA. 12h after siRNA transfection, medium was replaced with fresh medium containing TNFα (10ng/ml) or PBS. Samples were collected 10h after stimulation. Values represent means ± SEM of three different experiments performed in duplicate. * p < 0.05, ** p < 0.005, t-test.