γ-Secretase Modulators and APH1 Isoforms Modulate γ-Secretase Cleavage but Not Position of ε-Cleavage of the Amyloid Precursor Protein (APP)
Fig 2
Neither flurbiprofen nor fenofibrate shifted ε-cleavage position in a cell-free assay by MALDI-TOF.
Representative MALDI-TOF tracings of AICD peptides after incubation of crude membrane extracts of wild type APP expressing CHO cells at 37°C showed the presence of predominantly AICD50 fragment and minute amounts of AICD51 (A). Membrane preparations incubated with flurbiprofen (B) or fenofibrate (C) showed similar MALDI-TOF tracing as control. Inset: supernatant obtained from membranes shown in (A) incubated at 4°C and 37°C were analyzed by western blot. This confirmed that AICD50 was produced only following incubation at 37°C.