Diurnal Variations of Circulating Extracellular Vesicles Measured by Nano Flow Cytometry
Fig 2
EVs detected in plasma from a healthy donor.
Plasma from 5 mL of blood was collected, centrifuged to remove cellular debris (see methods), and imaged following a series of dilutions in PBS (A) to test for ‘swarming’. An EV population (based on positioning of 100 nm liposomes and size distribution of plasma samples) from the plasma of a healthy donor was sorted (gate R2; B) and imaged using atomic force microscopy (C). The size distribution of sorted EVs was analyzed by qNano and is represented in D. The gating strategy for these experiments is detailed in the methods section.