An Engineered Version of Human PON2 Opens the Way to Understand the Role of Its Post-Translational Modifications in Modulating Catalytic Activity
Fig 3
The PAO1 cells were grown o.n. and inoculated in a 96-well microplate in the presence and absence of rPON2 (50 and 100 μg/ml) and PON1 (100 μg/ml). PAO1 cells grown in presence of the enzyme storage buffer (20 mM pH 8.5 containing 0.2 mM Ca++) were used as control. After 48 h of incubation at 37°C, the medium was removed from the wells, and the biofilm formation was evaluated by crystal violet assay. The absorbance of each well was read at 540 nm. Biofilm formation was reported as a percentage in comparison with to the maximum amount of biofilm produced by POA1 cells grown in absence of enzymes (positive control). Each condition was made in six different wells and the experiment was carried out twice. Results are the average of duplicate and error bars show the range of the duplicate.