Widespread Myocardial Delivery of Heart-Derived Stem Cells by Nonocclusive Triple-Vessel Intracoronary Infusion in Porcine Ischemic Cardiomyopathy: Superior Attenuation of Adverse Remodeling Documented by Magnetic Resonance Imaging and Histology
Fig 6
Vessel density and cardiomyocyte hypertrophy.
A. Representative immunostaining sections with sma, isolectin B4 and DAPI, 1 month post treatment. B. Vessel density within the IZ (p = 0.05), C., the BZ and D., the RZ in the CDC-treated compared to placebo (Scale bar = 75μm). E. Representative sections immunostained with α-sa and WGA. F., G. Similar cardiomyocyte diameters in all three groups, both in the BZ and in the RZ. Scale bar = 50μm. Abbreviations: IZ, infarct zone; BZ, border zone; RZ, remote zone; sma, smooth muscle actin (red); isolectin B4 (green); DAPI, blue, 4',6-diamidino-2-phenylindole; asa, α-sarcomeric actinin; WGA, wheat germ agglutinin to define the cell borders. Error bars indicate SEM.