microRNA miR-142-3p Inhibits Breast Cancer Cell Invasiveness by Synchronous Targeting of WASL, Integrin Alpha V, and Additional Cytoskeletal Elements
Fig 8
siRNA-mediated depletion of WASL or ITGAV differentially affect membrane protrusions, cell volume and dry mass of MDA-MB-468 cells.
MDA-MB-468 cells were subjected to control, ITGAV or WASL siRNA treatment, followed by analysis via AFM (a) or DHM (b,c), 72h after transfection. (a) MDA-MB-468 cell surfaces were imaged by Atomic Force Microscopy (AFM) at nanometer resolution. Quantitative analysis of object counts as a readout of membrane protrusion formation reveals a significant reduction in cells treated with WASL siRNA (** = p<0.01, n = 15, error bars = s.e.m.). (b,c) Digital holographic microscopy of siRNA transfected MDA-MB-468 cells reveals decreased cell volume (b) and dry mass (c) after ITGAV siRNA transfection compared to control siRNA-transfected cells. *P<0.05, N = 200 cells per group, data are mean ± SEM.