microRNA miR-142-3p Inhibits Breast Cancer Cell Invasiveness by Synchronous Targeting of WASL, Integrin Alpha V, and Additional Cytoskeletal Elements
Fig 2
Confirmation of differentially regulated mRNA targets in miR-142-3p vs control transfected breast cancer cell lines by qPCR.
Cells were transfected with a control miRNA, miR-142-3p or antimiR-142-3p as described in the methods section, followed by RNA and cDNA preparation and qPCR analysis. (a-i). Black columns = control, white columns = miR-142-3p precursor, N>6, error bars = SEM, #P = 0.052 (n.s.), *P<0.05, ***P<0.001. miR-142-3p upregulation inhibits expression of WASL (a), ITGAV (MDA-MB-231; MCF-7) (b), RAC1 (c), CFL2 (d), ROCK2 (MDA-MB-231 with P = 0.052, MDA-MB-468) (e), IL6ST (MDA-MB-231, MCF-7) (f), KLF4 (MDA-MB-468, MCF-7) (g), PGRMC2 (MDA-MB-468, MCF-7) (h), and ADCY9 (MDA-MB-468, MCF-7) (i).