The Role of M2 Macrophages in the Progression of Chronic Kidney Disease following Acute Kidney Injury
Fig 4
The impact of macrophage depletion on kidneys during the recovery phase.
(A) Treatment with LC during the recovery phase improved renal fibrosis (magnification, ×100) and it was associated with a significant reduction in (B) the expression of type IV collagen in kidneys and (C) the level of the pro-inflammatory cytokine IL-6, (n = 3–5 per group), (D) As seen by immunohistochemical staining, the TGF-β expression decreased significantly in the kidneys of clodronate-treated mice on day 28. The TGF-β mRNA expression in kidneys also significantly decreased following liposome clodronate injection during the recovery phase. Magnification: ×100, (n = 4–6 per group), *p<0.05 compared to sham, #p < 0.05 compared to IRI+PBS, †p < 0.05 compared to the previous time point.