Positive Allosteric Modulation of Kv Channels by Sevoflurane: Insights into the Structural Basis of Inhaled Anesthetic Action
Fig 10
Putative sevoflurane binding sites in the Kv1.2 channel.
(A) Representation of four distinct sevoflurane binding locations on Kv1.2: site 1 (light blue), 2 (blue), 3 (black) and 4 (purple). Each pair of subunits is represented in green and orange. Mutation G329T is highlighted yellow. (B) Close-up view of ligand binding sites and mutation. Note that site 2 is in close proximity to the mutated residue G329T. (C) Binding constants of individual sevoflurane sites. These estimates were obtained using the LIE method as described under S1 File. O and C stand for activation-open and resting-closed conformations of the channel.