CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo
Fig 4
Slx2 knockout did not affect cell distribution and XY body formation.
A)–E) Immunofluorescence staining of 14 weeks old male mice paraffin embedded testes sections and quantification. PLZF is a spermatogonia stem cell marker. SYCP3 is a marker of synaptonemal complex. γH2AX forms specific foci at pachytene cells. SLX2 was labeled in red; PLZF, SYCP3 and γH2AX were labeled in green; cell nuclear labeled by DAPI was in blue. F) Immunofluorescence staining of 53BP1, which indicates the XY body in pachytene cells. No conceivable abnormality can be observed. SYCP3 was labeled in red; 53BP1 was labeled in green. X chromosome was drawn in blue; Y chromosome was drawn in red; the XY body was outlined with green. N.S., not significant; WT, wild-type.