Endogenous Murine BST-2/Tetherin Is Not a Major Restriction Factor of Influenza A Virus Infection
Fig 1
BST-2 expression is upregulated on murine macrophages but not alveolar epithelial cells in response to influenza A virus.
Monolayers of (A) the LA-4 AEC line and primary AEC, or (B) RAW264.7 macrophages and primary macrophages were incubated (i) with a MOI of 5 (HKx31) for 1 hour at 37°C and washed to remove excess virus (IAV infection, solid black line), (ii) in 1000 IU/ml recombinant mouse IFNα (dashed line) or (iii) in media alone (no infection, grey histogram). Cells were then incubated at 37°C for a total of 4 or 24 hours and levels of cell-surface BST-2 determined by flow cytometry. For each cell type, the isotype control (solid black histograms) is shown for ‘no infection’ cells only but is representative of profiles obtained using IAV-infected and IFNα-treated cells. Data are representative of 3 independent experiments.