Syncytiotrophoblast Extracellular Vesicles from Pre-Eclampsia Placentas Differentially Affect Platelet Function
Fig 5
STBEV increase thrombus formation.
Whole blood mixed with the lipophilic dye 3,3-dihexyloxacarbocyanine iodide was treated with N STBEV, PE STBEV or Tyrodes-HEPES control and perfused through collagen-coated (400 μg/mL) Vena8Biochip at a shear rate of 20 dyn/cm2. Thrombi were recorded through a series of images in the Z-plane through their full depth every 30sec using a Nikon eclipse (TE2000-U) microscope, thrombus fluorescence intensity was calculated using Slidebook, Version 5. The data represent thrombus volume (A) and sum fluorescence (B) (total fluorescence) intensities and are the mean of 3 separate experiments on different blood donors. A post-hoc 2-way ANOVA with Bonferroni correction showed no significant difference between control and N STB EV, the rate of thrombus formation (rate of change) (C and D) was increased in PE STBEV vs control (*P≤0.05, ***P≤0.001, ****P≤0.0001) though there was no difference in the final thrombus size at 10 min. (E) 3D rendered images of thrombi formed under each experimental condition.