Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator
Fig 3
Purified recombinant EAChm stimulates acetyltransferase activity of Chameau.
A) Amino acid sequence of EAChm. Acidic residues D and E are indicated with a red letter. The peptide sequence identified by mass spectrometry is underlined. B) Expression of flag-tagged EAChm in E.coli. After purification, protein was subjected to 10% SDS—PAGE and then visualized by Coomassie brilliant blue R staining. C) Recombinant flag-tagged EAChm (rf-EAChm) enhances H3 acetyl transferase activity. HAT assay was performed in the presence of either native EAChm (n-EAChm) or rf-EAChm, Chameau (fraction 4 from glycerol gradient sedimentation) and [3H] acetyl-CoA using salt dialyzed chromatin.