Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer
Fig 1
Chemical structure and anti-cancer activities of Etoposide and C-10 on breast cancer cells.
(A) Chemical structure of Etoposide and C-10 [4β-[6-(3, 4-dichlorophenyl) 3, 4-dihydro-2-methyl-4-oxoquinazolin-6-yloxy) hexanamide]-4-desoxy-podophyllotoxin]. (B) Trypan blue assay for cell viability on MCF-7 and MDA-MB-231 cells showing gradual decrease in viability after treatment with Etoposide or C-10 at 1–16 μM or Cisplatin at 5–40 μM and incubated for 24 h. (C) HUVEC cells were grown on EGM media and treated with Cisplatin, Etoposide or C-10 compounds after tube formation. Drastic inhibitory effect was observed upon C-10 treatment. (D) Wound healing assay on MCF-7 and MDA-MB-231 cells showing antiangiogenic and antimigratory effects after treatment with C-10 for 48 h. Images were obtained at 0 h, 24 h and 48 h by using an inverted microscope with 4X objective lens.