Highly Invasive Listeria monocytogenes Strains Have Growth and Invasion Advantages in Strain Competition
Fig 3
Effect of strain competition on the invasion efficiency of L. monocytogenes strains.
Invasion efficiency (%) of L. monocytogenes strains (A) PL25-RifR, (B) C5-StrR, (C) ScottA-StrR/RifR and (D) 6179-RifR grown alone (single) or in the presence of a second L. monocytogenes strain (1 day, 10°C, TSB-Y) was determined using Caco2 cells. Cells were infected for 1h with bacteria (multiplicity of infection of 25), and incubated for 45min (invasion) with gentamycin. Data, represented as % of invasion, are mean values ± standard deviation of three biological replicates performed in triplicate. *indicates significant difference of the mixed culture compared to the corresponding single culture (P<0.05). p-values are shown in S3 Table.