New Isoform of Cardiac Myosin Light Chain Kinase and the Role of Cardiac Myosin Phosphorylation in α1-Adrenoceptor Mediated Inotropic Response
Fig 3
(A) Phosphorylation levels of MLC2v determined by urea-glycerol-PAGE in C57BL/6J and C57BL/6N hearts. The ratio (%) of phosphorylated MLC2v vs. total MLC2v was calculated. The expression of smMLCK (B), ZIPK (C) and MYPT2 (myosin phosphatase target subunit 2) (D) in C57BL/6J and C57BL/6N hearts. Upper panels show representative Western blots and the lower panels summarize the densitometrical data. The expression level in C57BL/6J was expressed as 100%. *P<0.05. n = 4.