Downregulation of Choline Kinase-Alpha Enhances Autophagy in Tamoxifen-Resistant Breast Cancer Cells
Fig 6
The autophagy enhanced by downregulation of Chk-α contributed to protect MCF-7/TAM cells from tamoxifen.
(A) MTT assay for cell viability analysis. Treatment with 3-methyladenine (3-MA), an autophagy inhibitor induced the cell death in MCF-7/TAM and MCF-7/TAM-shChk-α cells time-dependently. (B) Western blot analysis of the LC3. 3-MA led to a significant reduction of elevated LC3 in MCF-7/TAM-shChk-α cells after treatment with 3-MA. (B) Western blot analysis of the p62. 3-MA caused to a slight decrease in p62 level in MCF-7/TAM-shChk-α cells. Data are presented as the mean ± standard deviation of 5 independent experiments. *p<0.05. 3-MA vs untreated.